‹ Video: “Peptide ID and Protein Inference..” by Prof. Alexey Nesvizhskii (U. Michigan) •
Prof. Josh Elias (left) of Stanford University receives a thank-you gift from David Chiang after his talk.
Ever wondered about target-decoy searching? Want to gain a better understanding and realistic expectation of this effective tool? SageNResearch’s video “Addressing Peptide Identification Signal-to-noise With Target-Decoy Searching”, given by Professor Josh Elias of Stanford University at our “Translational Proteomics 2.0″ meeting, can help. Dr. Elias is an Assistant Professor in Chemical and Systems Biology at Stanford University, and was part of the Steven Gygi Lab at Harvard Medical School before that. His lab is keenly interested in developing and applying methods to meet the current challenges facing scientists engaged in large scale proteome characterization.
Josh kicked off his talk with a stunning and very powerful visual to hit home the concept of what target-decoy database searching can do — you’ll never look at coffee beans in quite the same way. With this talk, you’ll know how to better find a happy medium for thresholds, smarter ways of designing your filtering criteria, when not to even consider using the method, how to get the most out of (really easy) decoy searching in SORCERER, and what’s so good about partial tryptic searches.
The 30-minute presentation is available at: http://www.scivee.tv/node/15544
To view slides, I recommend using the “full screen” mode. The slide set can also be downloaded as a Powerpoint file.